England coach Eddie Jones has dropped George Kruis for the visit of Australia to Twickenham Saturday.
Kruis played 57 minutes of England's win over Argentina, but is one of nine players released back to their clubs for Aviva Premiership action.
British & Irish Lions lock Kruis returns to Saracens alongside Nick Isiekwe and Alex Lozowski.
Leicester prop Ellis Genge has also been released, meaning Joe Marler -- suspended for the Pumas' visit -- will be involved in the 23-man squad Jones will name Thursday.
Tom Dunn, Piers Francis, Zach Mercer, Marcus Smith and Denny Solomona have all also returned to their clubs.
Mike Brown, who is completing his return to play protocols having suffered a concussion against Argentina, is one of the 25 players retained by Jones.
England squad:
Backs: Brown, Daly, May, Rokoduguni, Watson, Care, Farrell, Ford, Joseph, Slade, Youngs.
Forwards: Cole, Ewels, George, Hartley, Hughes, Itoje, Launchbury, Lawes, Marler, Robshaw, Simmonds, Underhill, Vunipola, Williams.