Maro Itoje has not been included in the England 31-man training squad ahead of Saturday's final Six Nations match against Scotland at Twickenham.
The Saracens forward sustained a knee injury in the championship opener against Ireland at the Aviva Stadium on Feb. 2.
The Rugby Football Union (RFU) confirmed in a statement that Itoje has returned to Saracens to continue rehabilitation after missing the 57-14 victory over Italy Saturday.
England head coach Eddie Jones has included a quartet of uncapped players with Ben Earl, Elliott Stooke, Marcus Smith and Ollie Thorley involved with the group.
England need to win and hope Wales slip up to stand any chance of winning the Six Nations title next weekend.
Wales could clinch the Grand Slam with a win at home over Ireland before England kick off at Twickenham.
Full England squad:
Forwards (17): Dan Cole, Luke Cowan-Dickie, Tom Curry, Ben Earl, Charlie Ewels, Ellis Genge, Jamie George, Nathan Hughes, George Kruis, Joe Launchbury, Ben Moon, Brad Shields, Kyle Sinckler, Elliott Stooke, Billy Vunipola, Harry Williams, Mark Wilson.
Backs (14): Joe Cokanasiga, Elliot Daly, Owen Farrell (captain), George Ford, Jonathan Joseph, Jonny May, Jack Nowell, Dan Robson, Henry Slade, Marcus Smith, Ollie Thorley, Ben Te'o, Manu Tuilagi, Ben Youngs.