Jonny Gray has not recovered from a shoulder injury in time for Scotland's final Six Nations game against France on Friday while Sean Maitland also misses out, Scottish Rugby said.
Gray was excluded from the 29-man squad named by coach Gregor Townsend for the trip to the Stade de France. The influential second rower had been hurt against Ireland and missed Saturday's win over Italy.
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Maitland, who started at full-back in the 52-10 win over Italy, was also left out for the rearranged match, which had been postponed after a COVID-19 outbreak in the French camp.
Scottish media said the absence of Saracens winger Maitland was due to a limited number of England-based players being made available to the Scots as the game is being played outside the international window.
Captain Stuart Hogg, centre Chris Harris, scrum-half Scott Steele, and locks Sam Skinner and Alex Craig are the five English-based players retained in the Scottish squad.