| MLB First Round Draft Picks - 1965 | ||||
| PICK | PLAYER | POS | TEAM | SCHOOL/HOMETOWN |
| 1 | Rick Monday | OF | Kansas City Athletics | Arizona State U |
| 2 | Les Rohr | LHP | New York Mets | Billings, MT |
| 3 | Joe Coleman | RHP | Washington Senators | Natick, MA |
| 4 | Alex Barrett | SS | Houston Astros | Winton, CA |
| 5 | Billy Conigliaro | OF | Boston Red Sox | Swampscott, MA |
| 6 | Rick James | RHP | Chicago Cubs | Florence, AL |
| 7 | Ray Fosse | C | Cleveland Indians | Marion, IL |
| 8 | John Wyatt | SS | Los Angeles Dodgers | Bakersfield, CA |
| 9 | *-Eddie Leon | SS | Minnesota Twins | U of Arizona |
| 10 | Doug Dickerson | OF | Pittsburgh Pirates | Birmingham, AL |
| 11 | Jim Spencer | 1B | California Angels | Glen Burnie, MD |
| 12 | Dick Grant | 1B | Milwaukee Braves | Watertown, MA |
| 13 | Gene Lamont | C | Detroit Tigers | Kirkland, IL |
| 14 | Al Gallagher | 3B | San Francisco Giants | Santa Clara U |
| 15 | Scott McDonald | RHP | Baltimore Orioles | Yakima, WA |
| 16 | Bernie Carbo | OF | Cincinnati Reds | Garden City, MI |
| 17 | Ken Plesha | C | Chicago White Sox | Notre Dame U |
| 18 | *-Mike Adamson | RHP | Philadelphia Phillies | San Diego, CA |
| 19 | Bill Burbach | RHP | New York Yankees | Dickeyville, WI |
| 20 | Joe DiFabio | RHP | St. Louis Cardinals | Delta State U |