Eddie Jones has left out four players who toured with the British & Irish Lions in his latest England training squad ahead of the autumn internationals.
Jonathan Joseph, the Bath outside centre, has been omitted from the 33-man squad, while James Haskell, Kyle Sinckler and George Kruis are also left out.
Jones has called up two uncapped players with Bath hooker Tom Dunn and Harlequins fly-half Marcus Smith named. The squad meet on Sunday for a three-day camp in Oxford but there is no place for Sale's Denny Solomona, who was sent home from their Teddington gathering, nor Bath winger Semesa Rokoduguni, who has made an impressive start to the season.
There is a familiar feel to a number of players included with captain Dylan Hartley one of three hookers alongside Jamie George and Dunn while Jones keeps the faith with Harry Williams, who made his debut against Argentina, with Sinckler left out.
With Kruis omitted, Joe Launchbury, Nick Isiekwe, Maro Itoje, Courtney Lawes and Charlie Ewels are the second-rows named with Chris Robshaw, Sam Underhill, Nathan Hughes, Tom Curry and Billy Vunipola covering the back-row.
Jones has named just two scrum-halves in Danny Care and Ben Youngs while 18-year-old Smith joins George Ford, Owen Farrell, Alex Lozowski and Piers Francis as No.10 options. Bar the surprise of Joseph's omission, the other backs have all featured under Jones.
"This camp in Oxford is a really positive opportunity for the group to come back together and sharpen our focus and preparation ahead of our first match against Argentina," Jones said.
"This is our most important game this year and we want the players to come away from camp having trained well with a clear plan of how we want to play and win against the Pumas."
England squad
Forwards: Dan Cole (Leicester Tigers), Tom Curry (Sale Sharks), Tom Dunn (Bath Rugby), Charlie Ewels (Bath Rugby), Ellis Genge (Leicester Tigers), Jamie George (Saracens), Dylan Hartley (Northampton Saints), Nathan Hughes (Wasps), Nick Isiekwe (Saracens), Maro Itoje (Saracens), Joe Launchbury (Wasps), Courtney Lawes (Northampton Saints), Joe Marler (Harlequins), Chris Robshaw (Harlequins), Sam Underhill (Bath Rugby), Harry Williams (Exeter Chiefs), Billy Vunipola (Saracens), Mako Vunipola (Saracens)
Backs: Mike Brown (Harlequins), Danny Care (Harlequins), Elliot Daly (Wasps), Owen Farrell (Saracens), George Ford (Leicester Tigers), Piers Francis (Northampton Saints), Alex Lozowski (Saracens), Jonny May (Leicester Tigers), Jack Nowell (Exeter Chiefs), Henry Slade (Exeter Chiefs), Marcus Smith (Harlequins), Ben Te'o (Worcester Warriors), Anthony Watson (Bath Rugby), Marland Yarde (Harlequins), Ben Youngs (Leicester Tigers)