Gloucester fly-half Danny Cipriani has been left out of head coach Eddie Jones' third World Cup training camp in Treviso, Italy.
Cipriani was included in the previous training squad selection but spent time away from the team to do specialised strength and conditioning work.
The 31-year-old -- who was named Premiership player of the year and RPA players' player last season -- has scored 64 points from his 16 international caps but has not played for England since June 2018 when he was influential in a test match win over South Africa.
Jones has picked a 38-man squad ahead of the 12-day training camp, including uncapped Harlequins centre Joe Marchant, who will join the group for the first time.
"Treviso is a hard yards camp with conditions similar to Tokyo," Jones said in a statement. "Team training will be about being adaptable and off the field enjoying each other's company."
"Selection is absolutely challenging, we have left some good players doing work at their clubs and they must be ready if called up."
The warm weather training camp is aiming to help acclimatise the players to conditions expected during the tournament in Japan in October.
The deadline for Jones to select his final 31-man squad for the tournament is Sept 2.
England's next games will be against Wales and Ireland at Twickenham in August, and Italy in Newcastle on Sept 6, as part of the Quilter Internationals ahead of the start of the Rugby World Cup in Japan against Tonga on Sept 22.
England training camp squad:
Forwards: Dan Cole (Leicester Tigers), Luke Cowan-Dickie (Exeter Chiefs), Tom Curry (Sale Sharks), Charlie Ewels (Bath Rugby), Ellis Genge (Leicester Tigers), Jamie George (Saracens), Maro Itoje (Saracens), George Kruis (Saracens), Joe Launchbury (Wasps), Courtney Lawes (Northampton Saints), Lewis Ludlam (Northampton Saints), Joe Marler (Harlequins), Brad Shields (Wasps), Kyle Sinckler (Harlequins), Jack Singleton (Saracens), Sam Underhill (Bath Rugby), Billy Vunipola (Saracens), Mako Vunipola (Saracens), Harry Williams (Exeter Chiefs), Mark Wilson (Sale Sharks).
Backs: Mike Brown (Harlequins) Joe Cokanasiga (Bath Rugby), Elliot Daly (Saracens), Owen Farrell (Saracens), George Ford (Leicester Tigers), Piers Francis (Northampton Saints), Willi Heinz (Gloucester Rugby), Jonathan Joseph (Bath Rugby), Joe Marchant (Harlequins), Jonny May (Leicester Tigers), Ruaridh McConnochie (Bath Rugby), Jack Nowell (Exeter Chiefs), Henry Slade (Exeter Chiefs), Ben Spencer (Saracens), Ben Te'o (unattached) Manu Tuilagi (Leicester Tigers), Anthony Watson (Bath Rugby), Ben Youngs (Leicester Tigers).