Henry Slade, Sam Underhill and Ruaridh McConnochie will all miss England's World Cup warm-up Test against Wales on Sunday due to injury, Eddie Jones has confirmed.
Slade has suffered a knee injury, while Underhill and McConnochie will be absent with toe and hip injuries respectively, Jones said ahead of the Twickenham Test.
Joe Cokanasiga will replace Bath teammate McConnochie in the backline, with Jonathan Joseph and the uncapped Lewis Ludlam stepping in for Slade and Underhill.
Meanwhile, Courtney Lawes and Manu Tuilagi have been added to the replacements bench.
While McConnochie's injury sees him miss out on an England debut, scrum-half Willi Heinz will make his on Sunday.
England XV team to face Wales:
15. Elliot Daly, 14. Joe Cokanasiga, 13. Jonathan Joseph, 12. Piers Francis, 11. Anthony Watson, 10. George Ford (captain), 9. Willi Heinz, 1. Ellis Genge, 2. Luke Cowan-Dickie, 3. Dan Cole, 4. Joe Launchbury, 5.Charlie Ewels, 6. Lewis Ludlam, 7. Tom Curry, 8. Billy Vunipola
Replacements: 16. Jack Singleton, 17. Joe Marler, 18. Harry Williams, 19. George Kruis, 20. Courtney Lawes, 21. Ben Youngs, 22. Joe Marchant, 23. Manu Tuilagi
Wales XV to face England:
15. Liam Williams, 14. George North, 13. Jonathan Davies, 12. Hadleigh Parkes,11. Josh Adams, 10. Gareth Anscombe, 9. Gareth Davies, 1. Nicky Smith, 2. Ken Owens, 3. Tomas Francis, 4. Adam Beard, 5. Alun Wyn Jones, 6. Aaron Wainwright, 7. Justin Tipuric, 8. Ross Moriarty
Replacements: 16. Elliot Dee, 17. Wyn Jones, 18. Dillon Lewis, 19. Jake Ball, 20. Aaron Shingler, 21. Tomos Williams, 22. Dan Biggar, 23. Owen Watkin.