Republic of Ireland defender Marc Wilson has been ruled out of this summer's European Championship campaign through injury.
Manager Martin O'Neill confirmed that the 28-year-old, who was named in the initial 35-man squad, will now not be available as he continues to fight his way back from a long-standing knee problem.
O'Neill told a news conference in Dublin on Monday: "Marc Wilson has had a setback. The best thing for him is to take four or five weeks off. He will be out."
MON: Marc Wilson has had a setback. The best thing for him is to take 4 or 5 weeks off. He will be out (of #EURO2016).. #COYBIG
- FAIreland (@FAIreland) May 23, 2016
Wilson has not played a senior game for club Stoke CIty since damaging knee ligaments in January in an FA Cup clash against Crystal Palace, and limped out of a behind-closed-doors friendly last month.
He last played for Ireland as a substitute in the 1-1 play-off draw with Bosnia-Herzegovina in Zenica in November.
Everton midfielder James McCarthy is to have a scan, but O'Neill has trimmed down his squad ahead of Friday night's friendly against Holland with Paul McShane, Alex Pearce, Anthony Pilkington and Jonathan Hayes all on standby.