"He wanted the ball like he always does," Astros manager A.J. Hinch said. "I'm sure it was emotional for him."
Asked about the fan reaction, Hinch said it was "pretty expected."
Osuna declined to speak on the advice of his lawyer. His case resumes Tuesday in Toronto.
Blue Jays manager John Gibbons called it "weird" to see Osuna on the mound at Rogers Centre wearing a different uniform. Osuna was traded to the Astros on July 30.
"Kind of mixed emotions, but I knew there was a good chance he was probably going to get it done," Gibbons said. "I've seen him do it too many times."
The Associated Press contributed to this report.