Frenkie de Jong will miss the last six games of Barcelona's season after sustaining another ankle injury in Sunday's 3-2 Clásico defeat against Real Madrid, a source confirmed to ESPN.
De Jong, 26, was taken off just before half-time after contesting the ball with Madrid midfielder Federico Valverde.
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The Netherlands international will not feature again this season, but a source told ESPN he should be fit for this summer's European Championship in Germany.
The Netherlands kick off their tournament against Poland on June 16, with games against France and Austria to follow in the group stage.
It is the third time this season De Jong has suffered an ankle injury.
He missed 10 games between September and November after spraining his ankle against Celta Vigo and another four matches in March with a similar problem.
"The injuries have been a bit unlucky," De Jong told ESPN before the Clásico on Friday.
"The first one someone fell on my ankle which left me with a syndesmosis thing which is a tough injury and then I had an injury on the same ankle again [in March] which was a tough blow."
Defeat at the weekend left Barça 11 points behind LaLiga leaders Madrid with just 11 games to go.
The loss followed Champions League elimination against Paris Saint-Germain in midweek.
With the team already out of the Spanish Super Cup and the Copa del Rey, they are likely to end the season trophyless as they relinquish the LaLiga title they won last year to Madrid.
Barça still need to finish second, though, to guarantee their participation in next season's Super Cup, with Girona just two points behind them in third.
Valencia are next up for the Blaugrana on April 29, with Xavi Hernández's side closing the campaign with fixtures against Girona, Real Sociedad, Almería, Rayo Vallecano and Sevilla